Extreme thermostability and strong strand-displacement activity enable applications unachievable in a single, closed-tube reaction using alternative isothermal enzymes.
Uniquely thermostable to 95oC for 5 minutes
Isothermal mplification of DNA
Extreme thermostability* (95ºC for 5 minutes)
Strong strand-displacement activity
65ºC temperature optimum
Suitable for HD-LAMP (Heat-denaturing LAMP).
5’-3’ exonuclease minus, 3’-5’ exonuclease minus
Supplied with 10X iBuffer 2
*no loss of activity is observed in a LAMP reaction following 5 minutes @ 95oC.
| Cat. No: | Description |
| TIN-001 | 5000U Tin(exo-) DNA Pol I |
| TIN-002 | 25,000U GspSSD DNA Pol I |
| TIN-003 | 100,000U GspSSD DNA Pol I |
| TIN-custom | Contact us for additional enzyme variations |
All pack sizes are available in the following standard concentrations:
8U/ul, 100U/ul (HC), 1000U/ul (EHC), 3000U/ul (UHC)
Enzymes are supplied with separate stocks of 10X iBuffer (no MgSO4),
5M Betaine and 50mM MgSO4 to enable fully customisable options in LAMP and RT-LAMP reaction setup.
Advantages of HD-LAMP:
- Template denaturation step at 95ºC prior to amplification has been shown to increase target sensitivity1
- Increased primer specificity – reduces false-priming that may occur at lower temperatures.
- Simple template extraction (cell-lysis) prior to 65ºC LAMP.
- Sample heat-lysis, amplification and detection in a single, closed tube.
1Mori Y, Notomi T. 2009. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemotherapy. 15(2):62-9.
