Contains our patented Tin(exo-) DNA Polymerase I with strong strand-displacement activity.
Uniquely thermostable to 95ºC for 5 minutes
65ºC temperature optimum
Suitable for HD-LAMP (Heat-denaturing LAMP)
5’-3’ exonuclease minus, 3’-5’ exonuclease minus
Lyophilised versions available (>1 year ambient stability)
LYO reagents available
OEM and customised solutions available
Supplied with our proprietary dsDNA intercalating dye as standard, no-dye (ND) kits also available.
| Cat. No: | Description |
| ISO-001TIN | Tin(exo-) Isothermal Mastermix 400 reactions (8X 50 reaction tubes) |
| ISO-DR001TIN | Tin(exo-) Isothermal Mastermix - Dried reagents 300 reactions (2X 150 reaction bottles, supplied with rehydration buffer) |
| ISO-001TIN-LYO-1K | LYO mastermix 1000 reactions (Base LYO mix, Tin(exo-) DNA Pol I, re-hydration buffer) |
| ISO-001TIN-custom | Contact us for additional mastermix variations |
Advantages of HD-LAMP:
- Template denaturation step at 95ºC prior to amplification has been shown to increase target sensitivity1
- Increased primer specificity – reduces false-priming that may occur at lower temperatures.
- Simple template extraction (cell-lysis) prior to 65ºC LAMP.
- Sample heat-lysis, amplification and detection in a single, closed tube.
1Mori Y, Notomi T. 2009. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemotherapy. 15(2):62-9.
