Renowned for its superior speed, this mastermix is ideal for swift isothermal amplification of DNA, with the added capability of native reverse-transcriptase activity facilitating single-enzyme RT-LAMP.
OptiGene’s Isothermal Master Mixes offer unrivalled DNA amplification speed
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) were compared to NEB’s WarmStart LAMP Kit (DNA & RNA)
A DNA target (VKORC1) was amplified from Human gDNA (Sigma). 25μl LAMP reactions were performed at 65ºC for 60 minutes on a real-time thermocycler (Roche LC-480) in duplicate. The ‘Time to result’ is defined as the time at which the amplification fluorescence value reaches a threshold of 10% of maximal fluorescence.
LAMP primers targeting the VKORC1 gene were designed using LAMPDesigner®.
0.8uM FIP – CTGTTGCACCTACCCTTCTGGAGCAGGAGAGGGAAATATCA
0.8uM BIP – GAAGTCAAGCAAGAGAAGACCTGAAAACTCCTGACCTCAAGTGA
0.2uM F3 – ACCATTATTCTGTCTACCACAC
0.2uM B3 – GACAGGGTTTCACCATGTT
0.4uM LoopF – TCTCTTCCTCTGGCGTCT
0.4uM LoopB – CTCACGCCTATAATCCTAGCATT
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) show faster DNA detection when compared to the NEB Master Mix.
OptiGene’s Isothermal Master Mixes offer unrivalled RNA amplification speed
GspSSD vs competitor ‘N’ (LAMP Pol 3.0) and competitor ‘L’ (thermostable LAMP Pol)
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) were compared to NEB’s WarmStart LAMP Kit (DNA & RNA) [Lot:0021609]. ISO-001 and ISO-004 DO NOT contain a specific reverse-transcriptase (RT) enzyme. For a true comparison to the NEB mix (which contains a separate and specific reverse-transcriptase enzyme) 0.5U AMV-RT was added to the OptiGene Master Mix reactions (ISO-001 inc. RT and ISO-004 inc. RT). Standard ISO-001 and ISO-004 RT-LAMP reactions were also compared to show the excellent inherent reverse-transcriptase activity of the mix.
A RNA target (Beta-actin) was amplified from Human Total RNA (Ambion). 25μl RT-LAMP reactions were performed at 65ºC for 60 minutes on a real-time thermocycler (Roche LC-480) in duplicate. The ‘Time to result’ is defined as the time at which the amplification fluorescence value reaches a threshold of 10% of maximal fluorescence.
LAMP primers targeting the Beta-actin gene were designed using LAMPDesigner®.
0.8uM FIP – GAGCCACACGCAGCTCATTGTACACGGCATCGTCACCAAC
0.8uM BIP – CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC
0.2uM F3 – CCCTGAAGTACCCCATCGA
0.2uM B3 – ACAGCCTGGATAGCAACGT
0.4uM LoopF – GCCAGATTTTCTCCATGTCGTC
0.4uM LoopB – CGAGAAGATGACCCAGATCATGT
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) show excellent speed in RT-LAMP. ISO-004 amplified 2X faster than the NEB mix, and almost 3X faster if a specific RT enzyme is added for a like-for-like comparison.
This DNA and RNA amplification method boasts the fastest speed while demonstrating superior strand-displacement activity at its 65ºC temperature optimum. Additionally, it features exonuclease activities in both 5’-3’ and 3’-5’ directions, ensuring high fidelity throughout the process.
| Cat. No: | Primary Application |
| ISO-001 | GspSSD Isothermal Master Mix - 400 reactions |
| ISO-001nd | GspSSD Isothermal Master Mix, no dye - 400 reactions |
| ISO-001LNL | GspSSD Lyse’n’LAMP Isothermal Master Mix - 400 reactions |
| ISO-DR001 | GspSSD Isothermal Master Mix - Dried reagents – 300 reactions |
| ISO-002 | GspSSD Isothermal Master Mix - 2000 reactions - 5 x 400 reactions |
| ISO-002nd | GspSSD Isothermal Master Mix, no dye - 2000 reactions - 5 x 400 reactions |
| ISO-DR001-RT100 | Isothermal Reverse Transcriptase Master Mix - Dried reagents - 10 x 10 reactions |
| ISO-DR001-RT300 | Isothermal Reverse Transcriptase Master Mix - Dried reagents - 6 x 50 reactions |
| ISO-001-RT400 | Isothermal Reverse Transcriptase Master Mix - wet reagents - 8 x 50 reactions |
