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The Genie^® II is specifically designed to run any isothermal amplification method that employs target detection by fluorescence measurement. Genie^® II will detect all dyes that can be excited from a blue light source and with an emission above 510 nm. Further uses include enzyme kinetic analysis and protein denaturation analysis using fluorescent dyes.

Genie^® II will run for a full working day from the internal battery.

From empty Genie^® II will charge fully in less than 3 hours.

Each block can be used independently, Different protocols can be run on the two blocks and they can be started and end at different times. When optimisation of a new isothermal assay is required it is possible to run with a thermal gradient along a block so that each well is at a different temperature.

Genie^® II uses a proprietary tube strip that has a locking cap and maximises both optical and thermal efficiencies. Other tubes cannot be used with the Genie II instrument.

The temperature control system within Genie^® II works over a temperature range of 40°C to 99°C.

Software and firmware updates are available from our website and can easily be applied using the update function on Genie^® II.

Genie^® II will detect all dyes that can be excited from a blue light source and with an emission above 510 nm.

There are many Isothermal amplification methods and nearly all can be used on the Genie^® II system. RPA, NASBA, SMAP and BART have all been run successfully.

LAMP requires 6 primers with 8 specificities, we developed LAMP designer to offer an easy alternative and allow rapid and easy design.

Performing an annealing step, rather than a melt, on the finished amplification product results in a single product peak from which a Ta value can be extracted. Similar to a melt curve, the annealing Ta value is unique to the sequence amplified, confirming amplification of the correct product.

It is not mandatory to do a 95°C preheat prior to amplification but this can be included in the thermal protocol if required.

OptiGene’s proprietary enzyme along with Magnesium and buffers in the correct ratios to allow the user to quickly produce the best results from their Isothermal assay.

Isothermal Mastermix – ISO-001: This isothermal amplification mix allows fluorescence detection of the product on the Genie^® II platform but may also be used on generic qPCR instrumentation. An anneal curve can then be generated to confirm the product. This eliminates the need for gel electrophoresis or turbidity detection and allows for a closed-tube system.

Isothermal Mastermix, no dye – ISO-001nd: This isothermal amplification mix allows the addition of alternative dyes or probes for use on a Genie^® II. It could alternately be used for end-point gel electrophoresis detection where contamination is not an issue. Isothermal Mastermix, no dye, no pyrophosphatase – ISO-001nd-npy: This isothermal amplification mix is suitable for turbidometric detection. All OptiGene Isothermal Mastermixes are fully licensed with the Eiken Chemical Company for use in LAMP reactions. OptiGene Isothermal Mastermix can be used with RCA, SMAP and many other amplification technologies.

The polymerase chain reaction (PCR) relies upon thermal cycling to achieve DNA strand separation, primer annealing and finally target extension. Loop-mediated isothermal DNA amplification (LAMP) uses a DNA polymerase enzyme with strand displacement activity to achieve strand deparation, primer annealing and target extension at a single (isothermal) temperature.

Loop-mediated isothermal DNA amplification (LAMP) is a highly sensitive and specific nucleic acid amplification technique. For more info: www.loopamp.eiken.co.jp/e/lamp/index

For highest target specificity and sensitivity we recommend purifying the target DNA/RNA. The strand displacing enzymes offered by OptiGene are less susceptible to inhibitors compared to standard PCR enzymes (e.g. Taq) and as such may need little/no template purification.

The Genie instrument is designed to be used by anyone with basic training. Simple reaction set up with easy to use touchscreen operation facilitates molecular detection for all.

For optimal LAMP primer design we recommend LAMPDesigner^® software.

We recommend running control reactions (positive and negative) for all laboratory techniques.