GspSSD Isothermal Mastermix (ISO-001)
- OptiGene’s original mastermix for isothermal amplification – just add template and primers.
- Contains OptiGene’s proprietary novel GspSSD LF DNA Polymerase.
- Superior amplification speed.
- Suitable for rapid isothermal amplification of DNA.
- Native reverse-transcriptase activity enables single enzyme RT-LAMP.
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OptiGene’s Isothermal Master Mixes offer unrivalled DNA amplification speed
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) were compared to NEB’s WarmStart LAMP Kit (DNA & RNA)
A DNA target (VKORC1) was amplified from Human gDNA (Sigma). 25μl LAMP reactions were performed at 65ºC for 60 minutes on a real-time thermocycler (Roche LC-480) in duplicate. The ‘Time to result’ is defined as the time at which the amplification fluorescence value reaches a threshold of 10% of maximal fluorescence.
LAMP primers targeting the VKORC1 gene were designed using LAMPDesigner®.
0.8uM FIP – CTGTTGCACCTACCCTTCTGGAGCAGGAGAGGGAAATATCA
0.8uM BIP – GAAGTCAAGCAAGAGAAGACCTGAAAACTCCTGACCTCAAGTGA
0.2uM F3 – ACCATTATTCTGTCTACCACAC
0.2uM B3 – GACAGGGTTTCACCATGTT
0.4uM LoopF – TCTCTTCCTCTGGCGTCT
0.4uM LoopB – CTCACGCCTATAATCCTAGCATT
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) show faster DNA detection when compared to the NEB Master Mix.
OptiGene’s Isothermal Master Mixes offer unrivalled RNA amplification speed
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) were compared to NEB’s WarmStart LAMP Kit (DNA & RNA) [Lot:0021609]. ISO-001 and ISO-004 DO NOT contain a specific reverse-transcriptase (RT) enzyme. For a true comparison to the NEB mix (which contains a separate and specific reverse-transcriptase enzyme) 0.5U AMV-RT was added to the OptiGene Master Mix reactions (ISO-001 inc. RT and ISO-004 inc. RT). Standard ISO-001 and ISO-004 RT-LAMP reactions were also compared to show the excellent inherent reverse-transcriptase activity of the mix.
A RNA target (Beta-actin) was amplified from Human Total RNA (Ambion). 25μl RT-LAMP reactions were performed at 65ºC for 60 minutes on a real-time thermocycler (Roche LC-480) in duplicate. The ‘Time to result’ is defined as the time at which the amplification fluorescence value reaches a threshold of 10% of maximal fluorescence.
LAMP primers targeting the Beta-actin gene were designed using LAMPDesigner®.
0.8uM FIP – GAGCCACACGCAGCTCATTGTACACGGCATCGTCACCAAC
0.8uM BIP – CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC
0.2uM F3 – CCCTGAAGTACCCCATCGA
0.2uM B3 – ACAGCCTGGATAGCAACGT
0.4uM LoopF – GCCAGATTTTCTCCATGTCGTC
0.4uM LoopB – CGAGAAGATGACCCAGATCATGT
OptiGene’s Isothermal Master Mixes (ISO-001 and ISO-004) show excellent speed in RT-LAMP.
ISO-004 amplified 2X faster than the NEB mix, and almost 3X faster if a specific RT enzyme is added for a like-for-like comparison.
Key features:
- Fast amplification of DNA and RNA
- 65ºC temperature optimum
- 5’-3’ exonuclease minus
- 3’-5’ exonuclease minus
| Cat No: | Description |
|---|---|
| ISO-001 | GspSSD Isothermal Master Mix - 400 reactions |
| ISO-001nd | GspSSD Isothermal Master Mix, no dye - 400 reactions |
| ISO-001LNL | GspSSD Lyse’n’LAMP Isothermal Master Mix - 400 reactions |
| ISO-DR001 | GspSSD Isothermal Master Mix - Dried reagents – 300 reactions |
| ISO-002 | GspSSD Isothermal Master Mix - 2000 reactions - 5 x 400 reactions |
| ISO-002nd | GspSSD Isothermal Master Mix, no dye - 2000 reactions - 5 x 400 reactions |
| ISO-DR001-RT100 | Isothermal Reverse Transcriptase Master Mix - Dried reagents - 10 x 10 reactions |
| ISO-DR001-RT300 | Isothermal Reverse Transcriptase Master Mix - Dried reagents - 6 x 50 reactions |
| ISO-001-RT400 | Isothermal Reverse Transcriptase Master Mix - wet reagents - 8 x 50 reactions |
Click HERE to request a free sample.
