GspM3.0 LF DNA Polymerase
Key features:
Cat. No: | Description | |
---|---|---|
GSPM3-001 | 1600u | GspM3.0 DNA polymerase @ 8u/µl |
GSPM3-002 | 8000u | GspM3.0 DNA polymerase @ 8u/µl |
GSPM3-002HC | 8000u | GspM3.0 DNA polymerase @ 100u/µl |
GSPM3-003HC | 40000u | GspM3.0 DNA polymerase @ 100u/µl |
iBuffer2-001 | 2 x 1.5ml iBuffer 2 | |
iBuffer2-002 | 15ml iBuffer 2 |
GspM3.0 vs competitor ‘N’ (LAMP Pol 3.0) and competitor ‘L’ (thermostable LAMP Pol)
25µl LAMP reactions: 8U DNA Pol, 1X manufacturers supplied reaction buffer (‘GspM3.0’ – iBuffer5, ‘N’ – IsoPol II, ‘L’ – BufferC), ), dNTPs/MgSO4 and Betaine – as per manufacturers recommended concentrations, 100-10,000 copies Human gDNA (Sigma), 1X Human VKORC1 primer mix – designed using LAMPDesigner™
(0.8µM FIP 5’-CTGTTGCACCTACCCTTCTGGAGCAGGAGAG GGAAATATCA-3’, 0.8µM BIP 5’-GAAGTCAAGCAAGAGAAGACCT GAAAACTCCTGACCTCAAGTGA-3’, 0.2µM F3 5’-ACCATTATTCTGTCTACCACAC-3’, 0.2µM B3 5’-GACAGGGTTTCACCATGTT-3’, 0.4µM LoopF 5’-TCTCTTCCTCTGGCGTCT-3’, 0.4µM LoopB 5’-CTCACGCCTATAATCCTAGCATT-3’), 3.75ng fluorescent intercalating dye (V13-01184 Dyomics GmbH). ‘GspM3.0’, ‘N’ and ‘L’ reactions were run as per manufacturer’s instructions at 65ºC, 65ºC and 70ºC respectively on a Genie®II. A: Real-time amplification plots. B: Time-to-threshold values (the maximal rate of change in fluorescence over time) reported in graphical format for simple comparison.