Tin exo-LF DNA polymerase, isolated from Thermodesulfatator indicus, has strong strand displacement activity and is thermostable for 10 minutes at 93ºC.
The full length unmodified enzyme possesses both 5’-3’ exonuclease and 3’-5’ exonuclease activities. Tin exo-LF DNA polymerase has been engineered to remove the 5’-3’ exonuclease domain and inactivate the 3’-5’ exonuclease activity. The enzyme has no reverse transcriptase activity.
The thermostability of Tin LF DNA polymerase can be seen in the figure on the left. Tin LF DNA pol can be heated to 93oC for 10 minutes with no loss of activity observed. Template denaturation using a heat step prior to 65C isothermal amplification has been shown to increase target specificity and reduce overall amplification times.