Reverse Transcription LAMP

GspSSD LF DNA polymerase, as used in the ISO-001 master mix, has good reverse transcriptase (RT) activity. GspM2.0 DNA polymerase has poor RT activity whilst the unmodified GspM DNA polymerase has no RT activity.

The addition of 0.125u of AMV reverse transcriptase will enhance the RT activity further, decreasing the reaction time, but the overall assay sensitivity is identical with or without AMV.

A comparison between ISO-001, GspM, GspM2.0, Bst and Bst2.0 in a fluorescent detection human B-actin RT LAMP reaction, run on the Genie® II instrument, is shown in the figure to the left.

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