Plant Health & Pests Diagnostics

OptiGene is pleased to launch a range of kits for the detection of plant health and pests.

OptiGene is fully licensed to manufacture and sell kits for LAMP tests that are used to detect plant diseases and pests. Simple sample preparation, ease of use and fast running time combine to offer distinct advantage over competing technologies. This allows the user rapid diagnostics at point of application.

The kits have been developed in collaboration with Fera Science Ltd. and the optimised LAMP assays have been validated to EPPO Standard PM 7/98.

Our kits are available in the following format:

Plant Health Specific Mastermix – Contains mastermix with separate vial of primers in a ready to use reaction mix. Just add template and run. The mastermixes do not include extraction reagents, internal control, disposable loops or Genie® Strips. Pack size available of 50 reactions.

Related products available are:

• Plant Material Lysis Kit, which contains buffer, bijous and ball bearing for a rapid and simple lysis method.
• Plant control assay – Contains mastermix with separate vial of primers for Cytochrome Oxidase, ready to use, this is used as a control for some of our field based kits.
• Control DNA for use with Plant Health Specific mastermix (50 reactions – 105 copies/µl)

Products:

Liriomyza Leaf miners

Guignardia citricarpa

Dothistroma septosporum

Clavibacter michiganensis subsp. Sepedonicus

Chalara fraxinea

Botrytis cinerea

Demonstration of Plant Testing Kit:

 

 

Catalogue Numbers for kits:

Plant Health Extraction Kit
Contains reagents and consumables required to perform extraction from plant material.

Cat No:	Description
EXT-001	Plant Material Lysis KIt - 50 rxns

Control DNA
Control DNA for use with Plant Health Specific mastermix (50 rxns – 10E5 copies/μl)

Cat No:	Description
CD-ACMV-050DR	African Cassava Mosaic virus (ACMV), dried DNA Control
CD-ASOL-050	Alternaria solani DNA control
CD-BCIN-050	B.cinerea DNA Control
CD-BDO-050	Bactrocera dorsalis group DNA Control
CD-BNOI-050	Candidatus phytoplasma solani (Bois Noir) DNA Control
CD-BTA-050	Bemesia tabaci DNA Control
CD-CFR-050	Chalara fraxinae DNA Control
CD-CLAF-050	Candidatus liberibacter africanus DNA Control
CD-CLAS-050	Candidatus liberibacter asiaticus DNA Control
CD-CLSO-050	Candidatus liberibacter solanacearum DNA Control
CD-CMS-050	Clavibacter michiganensis subsp. sepedonicus DNA Control
CD-COX-050	COX DNA Control
CD-CTV-050	Citrus tristeza DNA Control
CD-DSEP-050	Dothistroma septosporum DNA
CD-EACMV-050DR	East African cassava mosaic virus (EACMV), dried DNA Control
CD-EAM-050	Erwinia amylovora DNA Control
CD-FCM-050	False Codling Moth DNA Control
CD-FDO-050	Flavescence doree DNA Control
CD-FFU-050	Fusarium fujikoroi DNA Control
CD-FLA-050	Fusarium lactucae DNA Control
CD-GCIT-050	Guignardia citricarpa DNA Control
CD-GCIT-050	Guardinia citricarpa DNA Control
CD-LHUI-050	Liriomyza huidobrensis DNA Control
CD-LIR-050	Liriomyza species DNA Control
CD-Liriomyza-050	Liriomyza DNA Control
CD-LSA-050	Liriomyza sativae DNA Control
CD-LTRI-050	Liriomyza trifolii DNA Control
CD-MFR-050	Monlinia fructicola DNA Control
CD-MLA-050	Monilinia laxa DNA Control
CD-MORZ-050	Magnaporthe oryzae DNA Control
CD-PKER-050	Phytophthora kernoviae DNA Control
CD-PRAM-050	Phytophthora ramorum DNA Control
CD-SDO-050	Scirtothrips dorsalis DNA Control
CD-TLE-050	Thaumatotibia leucotreta DNA Control
CD-TPA-050	Thrips palmi DNA Control
CD-XAR-050	Xanthomonas arboricola pv. pruni DNA Control
CD-XFA-050	Xylella fastidiosa DNA Control
CD-XFM-050	Xylella fastidiosa morus DNA Control
CD-XFS-050	Xylella fastidiosa sandyi DNA Control

Plant control assay
Contains mastermix with separate vial of primers for Cytochrome Oxidase, ready to use.

Cat No:	Description
PK-COX-050W	Cox master mix - 50rxns

Plant Health Specific Mastermix
Contains mastermix with separate vial of primers ready to use 50 reactions. Does not include extraction reagents, internal control, disposable loops or Genie® Strips.

Cat No:	Description
PK-A.solani-050W	Alternaria solani - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-ACMV-050DR	African Cassava Mosaic virus - 50rxns - 1 vial of 50rxns of dried master mix and 1vial of dried 50rxns of primer mix
PK-B.cinerea-050W	Botrytis cinerea master mix
PK-B.dorsalis-050W	Bactrocera dorsalis - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-B.tabaci-050W	Bemisia tabaci master mix
PK-C.lib.africanus-050W	Candidatus liberibacter africanus - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-C.lib.asiaticus-050W	Candidatus liberibacter asiaticus - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-C.lib.solanacearum-050W	Candidatus liberibacter solanacearum - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-C.mich.sepedonicus-050W	Clavibacter michiaganensis subsp. sepedonicus master mix
PK-C.tristeza-050W	Citrus tristeza virus - 50rxns - 1 vial of 50rxns of RT master mix and 1vial of 50rxns of primer mix
PK-Chalara-050W	Chalara fraxinea master mix
PK-D.septosporum-050W	D.septosporum master mix
PK-E.amylovora-050W	Erwinia amylovora master mix
PK-EACMV-050DR	East African cassava mosaic virus - 50rxns - 1 vial of 50rxns of dried master mix and 1vial of dried 50rxns of primer mix
PK-F.doree-050W	Flavescence dorée assay mix - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-F.fujikuro-050W	Fusarium fujikuro- 50rxns - 1 vial of 50rxns of master mix and 1 vial of 50rxns of primer mix
PK-F.oxy_lac-050W	Fusarium oxysporum f. sp. lactucae - 50rxns - 1 vial of 50rxns of master mix and 1 vial of 50rxns of primer mix
PK-F.oxysporum_R4-050W	Fusarium oxysporum f.sp. cubense - distinguishes between race 4 and sub-tropical race4 - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer-probe mix
PK-F.oxysporum-050W	Fusarium oxysporum f.sp. cubense race 4 and sub-tropical race4 - 50rxns - 1 vial of 50rxns of master mix and 1 vial of 50rxns of primer mix
PK-G.citricarpa-050TW	Guignardia citricapra (Citrus Black Spot) master mix
PK-L.asiaticus-050W	Liberibacter asiaticus - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-Ly.huidobernsis-050W	Liriomyza huidobernsis master mix
PK-Ly.sativae-050W	Liriomyza sativae master mix
PK-Ly.Species-050W	Liriomyza species - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-Ly.trifolii-050W	Liriomyza trifolii master mix
PK-Ly-Control-050W	Liriomyza positive control master mix
PK-M.oryzae-050W	Magnaporthe oryzae 50rxns - 1 vial of 50rxns of master mix and 1 vial of 50rxns of primer mix
PK-P.Kernoviae-050W	Phytophthora kernoviae master mix
PK-P.Ramorum -050W	Phytophthora ramorum master mix
PK-PVA-050W	Potato virus A - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix 1
PK-PVX-050W	Potato virus X - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix 1
PK-PVY-050W	Potato virus Y - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-S.dorsalis-050W	Scirtothrips dorsalis master mix
PK-T.leucotreta-050W	Thaumatotibia leucotreta - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-T.palmi-050W	Thrips palmi master mix
PK-X.arbori.pruni-050W	Xanthomonas arboricola pv. pruni master mix
PK-X.fast.morus-050W	Xylella fastidiosa morus - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-X.fast.sandyi-050W	Xylella fastidiosa sandyi - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix
PK-X.fastidiosa-050W	Xylella fastidiosa - 50rxns - 1 vial of 50rxns of master mix and 1vial of 50rxns of primer mix